![]() ![]() Trainees are expected to practice on their own and request additional training time if necessary. ![]() It will take approximately 10-12 hours to solidify the concepts learned during the course.Training is only provided for the analyzers. ![]() A large gap between initial training and follow-up sessions is not recommended.Software training is quite intensive and it will take repeated solo practice sessions to become proficient.WORK- FLOW™ must be completed before this step – time to complete the course should be factored into your planning.Hands-on training should only begin when you are almost ready to regularly run samples (3-4 weeks).Due to the busy nature of the flow cytometry facility, there is usually a 2-week wait time between a request for training and the first hands-on session.if you intend to acquire your data at another facility please seek instruction at that location. Training will only be provided for those investigators who become regular users of our facility.Training is only available to students and employees of the University of Toronto and its affiliated research institutes (within the University Health Network).Please contact us to discuss your plans to receive an estimate of training costs for your application. The average cost of training varies between $550 – 750+, depending on experimental complexity. Mandatory for all experimental assays with greater than 4 fluorescent parameters.This session may or may not be required for simple experimental set ups 1- 4 colours.Follow-Up Session 2 – 3 hours $200/person, held one-on-one with a dedicated instructor.Experiment standardization and data export will be covered.An instructor will guide you through the optimization of your own samples on an instrument.Follow-Up Session 1 – 3 hours $200/person, held one-on-one with a dedicated instructor, required.Elements of the acquisition software will be demonstrated with fluorescent beads including: detector selection, compensation, drawing analysis templates, and saving application specific settings.Covers a walk-through of the instrument start-up, quality control check, instrument cleaning, and shut-down procedures.Introduction to flow cytometers and using BD FACSDiva – 3 hours $150/person, held in groups of two.Improved interaction with BD ® Research Cloud (BD RC)- BD RC is a new tool for collaboration, experiment and data management, panel design and more.Spectral similarity scores-Calculate the cosine index for your actual data to evaluate reagent choices you made in panel design.Built-in ClusterExplorer-Display line charts, bar charts, heat maps and dimensionality reduction plots of clustered data in FlowJo ™ Software without the use of a plugin.Also, gates with the same name can now be linked together as ‘clones’ so that their position is synchronized.Automatically create quads and rectangle gates based on range gates, bi-sector gates or the data itself.Extract Autofluorescence without Autospill-Use an unstained cell data file to model autofluorescence for subtraction.Marker Enrichment Modeling (MEM)-Name your clusters based on their expression with a human-and machine-readable label.Embed-Forward propagate your clustering or dimensionality reduction results to new files with the same parameters.The latest release of FlowJo™ v10.9 Software
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